Nurmagomedova P.M., Abasova M.M., Djanbolatova D.N. The Effect of Temperature Stress on the Enzymes of Protein Breakdown in Thedigestive System of Rodents // Arid ecosystems. 2018. V. 24. № 4 (77). P. 75-79. | PDF

We investigated the activity of enzymes of protein breakdown in the liver of rodents under temperature stress-induced hyperthermia (42°C) conditions, which are very common for hyper-arid regions of arid territories. Temperature stress were caused in a special chamber, which was leaking water of temperature, necessary to increase/reduce the temperature of the animal’s body. The activity of neutral proteases (ANP) of the liver was reduced in both cases, regardless of the body temperature. However, the mechanisms leading to decrease of ANP during hyperthermia and hypothermia can vary, as the rate of decrease of enzyme activity at hyperthermia is several times higher than at hypothermia. During hyperthermia ANP indices at every temperature of incubation were significantly reduced as compared with the control as well as with hypothermia, except for the incubation temperature of 10°C. Energy of activation amounted to 20 kJ/mol in the range of 42-10°С, and in the interval of 42-20°С was 63.7 kJ/mol. In the same temperature range 42-20°С during hypothermia EA was 2 times lower and amounted to 31.8 kJ/mol, almost without change compared with the control of 31.2 kJ/mol.

Keywords: temperature stress, hypothermia, hyperthermia, enzymatic activity, neutral proteases.

DOI: 10.24411/1993-3916-2018-00038